Journal: Cells

Article Title: A Mouse Model to Study the Pathogenesis of γ-herpesviral Infections in Germinal Center B Cells.

doi: 10.3390/cells12242780

Figure Lengend Snippet: Figure 5. Virally induced constitutively active CD30 signaling results in more Cre-reporter+ cells within B cells. CAR//Cγ1-cre mice (dots) and LC30//Cγ1-cre mice (squares) were i.p. infected with MHV-68 hNGFR and splenic cells were analyzed 14 days post-infection. (A) Breeding scheme of LC30//Cγ1cre mice. The excision of the loxP-flanked stop cassette was followed by hCD2 reporter expression (Cre-reporter) and ligand-independent constitutively active CD30 signaling. (B) The splenic

Article Snippet: The CD19-PeVio770 (REA749), CD38-PeVio770 and human CD2 (hCD2)-PeVio770 (LT2) antibodies were all purchased from Miltenyi Biotech (Bergisch Gladbach, Germany).

Techniques: Infection, Expressing

Journal: bioRxiv

Article Title: Ångström Resolution with Flow Immunofluorescence Localization Microscopy (FILM)

doi: 10.64898/2025.12.16.694546

Figure Lengend Snippet: (A–C) FILM acquisition of binder probes that recognize CD2, ICAM-1 (CD54) in the pSMAC, and CD45 in the dSMAC zones (10 nm pixels with 3-pixel Gaussian blurring). For CD2 and CD45, APC-conjugated antibodies were used; for ICAM-1, a nanobody Fab conjugated with Vio ® 667 was used. Cyan boxed region shows the IF image (103.17 nm pixels) corresponding to the white boxed region. (D–F) Zoom-in of the respective boxed regions for the panels to the left. Localizations are shown as peak-normalized 2D Gaussians with color-coded precision according to the colorbar. (G–I) Zoom-in of the respective boxed regions for the panels to the immediate left. Localizations are shown as crosses (indicating ±1-σ uncertainty in x and y ) with color-coded precision according to the colorbar.

Article Snippet: α-tubulin (REA1136 REAfinityTM, h, APC, #130-119-541, Miltenyi Biotec), CD2 (REA972 REAfinityTM, h, APC, #130-116-150, Miltenyi Biotec), CD20 (REA780 REAfinityTM, h, APC, #130-111-339, Miltenyi Biotec), CD28 (REA612 REAfinityTM, h, APC, #130-118-343, Miltenyi Biotec), CD45 (REA1023 REAfinityTM, h/nhp, APC, #130-117-191, Miltenyi Biotec), CD54/ICAM-1 (REA266 REAfinityTM, h, APC, #130-120-711, Miltenyi Biotec), CD81 (REA513 REAfinityTM, h, APC, #130-119-787, Miltenyi Biotec), CD107a/LAMP1 (REA792 REAfinityTM, h, APC, #130-111-847, Miltenyi Biotec), IgD (REA740 REAfinityTM, h, APC, #130-110-644, Miltenyi Biotec), IgM (REAL689 REAdye_leaseTM, h, PE, #130-125-786, Miltenyi Biotec), Pan-Cytokeratin (REA1141 REAfinityTM, h, APC, #130-120-096, Miltenyi Biotec), β-catenin (REA480 REAfinityTM, h, APC, #130-124-444, Miltenyi Biotec), TCRα/β (REA652 REAfinityTM, h, APC, #130-113-535, Miltenyi Biotec), TOM22 (REA1185 REAfinityTM, APC, h/ms, #130-122-079, Miltenyi Biotec), DAPI Staining Solution (#130-111-570, Miltenyi Biotec), and ATTO 643 phalloidin (ATT-AD643-81, ATTO-TEC).

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Exploration of the mechanism of Taohong Siwu Decoction for the treatment of ischemic stroke based on CCL2/CCR2 axis

doi: 10.3389/fphar.2024.1428572

Figure Lengend Snippet: Expression of CD86 (M1) and CD206 (M2). (A) : Expression of CD86 (M1); (B) : Expression of CD206 (M2). # P < 0.05, ## P < 0.01 vs. sham; * p < 0.05, ** p < 0.01 vs. MCAO/R.

Article Snippet: A11042), were both purchased from MultiSciences, APC anti-Human CD206 (LW1218) was purchased from Elabscience, human TNF-α (July 2023), Human matrix metalloproteinase 9 (MMP-9) (July 2023), human IL-6 (July 2023), human IL-4 (July 2023), rat TNF-α (May 2023), rat MMP-9 (May 2023), rat IL-6 (May 2023), rat IL-4 (May 2023) were purchased from Quanzhou Ruixin Biotechnology Co., Ltd., claudin-5 (cat.no.

Techniques: Expressing

Journal: Cell Reports Medicine

Article Title: SARS-CoV-2-Specific T Cells Exhibit Phenotypic Features of Helper Function, Lack of Terminal Differentiation, and High Proliferation Potential

doi: 10.1016/j.xcrm.2020.100081

Figure Lengend Snippet:

Article Snippet: CD2 , Fluidigm , Cat#3151003B.

Techniques: Purification, Recombinant, Electron Microscopy, Virus, Control, Clinical Proteomics, Staining, Antibody Labeling, Software

Journal: Nature Communications

Article Title: PTPN2 copper-sensing relays copper level fluctuations into EGFR/CREB activation and associated CTR1 transcriptional repression

doi: 10.1038/s41467-024-50524-5

Figure Lengend Snippet: a Evaluation of the effects on EGR1 expression levels of 200 μM CuCl 2 (60 min treatment) following siRNA knockdown of various PTPs in A549 cells. Depicted are two interpretations of the results. ( top ) All fold-changes are reported relative to the negative control siRNA transfect (Neg.) without CuCl 2 supplementation. ( bottom ) Each result is reported as the fold-change between the denoted siRNA knockdown after 60 min 200 μM CuCl 2 supplementation and the siRNA knockdown by itself. ( n = 3 replicates per condition). Same box plot definitions as in Fig. . * denotes p < 0.05, ** denotes p < 0.01, and *** denotes p < 0.001 for two-sided t -test comparison between conditions. b p NPP ( p -nitrophenyl phosphate) assay of ΔPTPN1 PTP activity following addition of various amounts of Cu 1+ ( n = 12 replicates per condition). c Proteome profiler antibody array volcano plot depicting statistically significant increases in phosphorylation levels following HEK 293 T supplementation with 20 μM ZnCl 2 . ( n = 2 replicates for each conditio n ). d Changes in A549 EGR1 expression levels 60 min after ZnCl 2 supplementation. The fold-changes are overlayed on the fold-changes induced by CuCl 2 supplementation for reference. ( n = 2 replicates per condition). Same box plot definitions as in Fig. . e RNA-qPCR fold-changes of select transcripts 4 h post-supplementation of 500 nM CuGTSM or 5 µM CS1 relative to DMSO treated A549 cells. ( n = 3 replicates per condition). Data points correspond to biological replicates, p -values calculated as paired two-tailed t-test. Same box plot definitions as in Fig. . Figure 4a, b, d, and e source data are provided as a Source Data file, as are exact p -values for Fig. 4a, e. * denotes p < 0.05, ** denotes p < 0.01, and *** denotes p < 0.001 for two-sided t -test comparison between conditions.

Article Snippet: For Cu-GTSM (Cayman Chemical Company) and CS1 (MedChemExpress) treatments, the solid drugs were dissolved in DMSO to a final concentration of 1 mM.

Techniques: Expressing, Knockdown, Negative Control, Comparison, Activity Assay, Ab Array, Phospho-proteomics, Two Tailed Test